The estimation of heparin and similar substances in human blood and tissues using a combined biological and colorimetric method with paper electrophoretic studies.

Heparin and other acid mucopolysaccharides were extracted from various tissues by a modification of the method previously described (Bassiouni, 1953). The dye mucopolysaccharides complex obtained was resolved into its components with formamide: the acid mucopolysaccharides were precipitated with methyl alcohol in the presence of electrolyte (sodium chloride). A solution of the purified polysaccharide precipitate was bio-assayed for active heparin, and the remainder was either submitted to electrophoresis on paper